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M2 medium
sterility:sterile-filtered.
form:liquid.
storage condition:protect from light.
technique(s):cell culture | embryo: suitable.
impurities:endotoxin, tested.
components:phenol red: 0.0106 g/L;sodium pyruvate: 0.0363 g/L;HEPES: 5.42726 g/L;glucose: 1.0 g/L (Dextro);NaHCO3: 0.35 g/L.Application:
Recommended for manipulation of mouse embryos at ambient temperature. M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently.
M2 Medium is a further modification of M16 that substitutes HEPES buffer in place of some of the bicarbonate. M2 is used for collecting and handling embryos for prolonged periods outside a CO2 incubator.
